Capillary isoelectric focusing using an LKB 2127 tachophor isotachophoretic analyzer

Millicent Anne Firestone; Wolfgang Thormann

doi:10.1016/S0021-9673(00)94588-6

Capillary isoelectric focusing using an LKB 2127 tachophor isotachophoretic analyzer

Millicent Anne Firestone
, Wolfgang Thormann

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Capillary isoelectric focusing (CIEF) was carried out using a commercial capillary isotachophoretic analyzer, the LKB 2127 Tachopor. The unmodified instrument was found to be unsuitable for fast focusing, due to non-optimized boundary conditions at the capillary ends, the length of the separation tube, and insufficient cooling of the focusing trough. Simple column changes were implemented, resulting in significantly improved applicability of the instrument for CIEF. The impact of these modifications was examined via the analysis of protein mixtures in both simple buffers and in synthetic carrier ampholytes.

Original language	English
Pages (from-to)	309-315
Number of pages	7
Journal	Journal of Chromatography A
Volume	436
Issue number	C
DOIs	https://doi.org/10.1016/S0021-9673(00)94588-6
State	Published - 1988
Externally published	Yes

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title = "Capillary isoelectric focusing using an LKB 2127 tachophor isotachophoretic analyzer",

abstract = "Capillary isoelectric focusing (CIEF) was carried out using a commercial capillary isotachophoretic analyzer, the LKB 2127 Tachopor. The unmodified instrument was found to be unsuitable for fast focusing, due to non-optimized boundary conditions at the capillary ends, the length of the separation tube, and insufficient cooling of the focusing trough. Simple column changes were implemented, resulting in significantly improved applicability of the instrument for CIEF. The impact of these modifications was examined via the analysis of protein mixtures in both simple buffers and in synthetic carrier ampholytes.",

author = "Firestone, \{Millicent Anne\} and Wolfgang Thormann",

year = "1988",

doi = "10.1016/S0021-9673(00)94588-6",

language = "English",

volume = "436",

pages = "309--315",

journal = "Journal of Chromatography A",

issn = "0021-9673",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Capillary isoelectric focusing using an LKB 2127 tachophor isotachophoretic analyzer

AU - Firestone, Millicent Anne

AU - Thormann, Wolfgang

PY - 1988

Y1 - 1988

N2 - Capillary isoelectric focusing (CIEF) was carried out using a commercial capillary isotachophoretic analyzer, the LKB 2127 Tachopor. The unmodified instrument was found to be unsuitable for fast focusing, due to non-optimized boundary conditions at the capillary ends, the length of the separation tube, and insufficient cooling of the focusing trough. Simple column changes were implemented, resulting in significantly improved applicability of the instrument for CIEF. The impact of these modifications was examined via the analysis of protein mixtures in both simple buffers and in synthetic carrier ampholytes.

AB - Capillary isoelectric focusing (CIEF) was carried out using a commercial capillary isotachophoretic analyzer, the LKB 2127 Tachopor. The unmodified instrument was found to be unsuitable for fast focusing, due to non-optimized boundary conditions at the capillary ends, the length of the separation tube, and insufficient cooling of the focusing trough. Simple column changes were implemented, resulting in significantly improved applicability of the instrument for CIEF. The impact of these modifications was examined via the analysis of protein mixtures in both simple buffers and in synthetic carrier ampholytes.

UR - https://www.scopus.com/pages/publications/0342387453

U2 - 10.1016/S0021-9673(00)94588-6

DO - 10.1016/S0021-9673(00)94588-6

M3 - Article

SN - 0021-9673

VL - 436

SP - 309

EP - 315

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - C

ER -

Capillary isoelectric focusing using an LKB 2127 tachophor isotachophoretic analyzer

Abstract

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