TY - JOUR
T1 - GFP-related chromophores
T2 - photoisomerization, thermal reversion, and DNA labelling
AU - Faillace, Martin S.
AU - Dolgopolova, Ekaterina A.
AU - Ceballos, Noelia M.
AU - Ruiz Pereyra, E. Nahir
AU - Lanfri, Lucia
AU - Argüello, Gustavo A.
AU - Burgos Paci, Maximiliano
AU - Shustova, Natalia B.
AU - Peláez, Walter J.
PY - 2023/6/9
Y1 - 2023/6/9
N2 - Due to the pronounced effect of the confined environment on the photochemical properties of 4-hydroxybenzylidene imidazolinone (HBI), a GFP-related chromophore, imidazolidinone and imidazothiazolone analogues have been studied as fluorescent probes. Their photoisomerization and their thermal reversion were studied under 365-nm-irradiation, resulting in observation of an enthalpy-entropy compensation effect. Theoretical studies were carried out to shed light on the thermal reversion mechanism. Moreover, photophysical studies of benzylidene imidazothiazolone in the presence of dsDNA revealed fluorescence enhancement. The prepared compounds could be considered as a valuable tool for the detailed investigation of physicochemical, biochemical, or biological systems.
AB - Due to the pronounced effect of the confined environment on the photochemical properties of 4-hydroxybenzylidene imidazolinone (HBI), a GFP-related chromophore, imidazolidinone and imidazothiazolone analogues have been studied as fluorescent probes. Their photoisomerization and their thermal reversion were studied under 365-nm-irradiation, resulting in observation of an enthalpy-entropy compensation effect. Theoretical studies were carried out to shed light on the thermal reversion mechanism. Moreover, photophysical studies of benzylidene imidazothiazolone in the presence of dsDNA revealed fluorescence enhancement. The prepared compounds could be considered as a valuable tool for the detailed investigation of physicochemical, biochemical, or biological systems.
UR - http://www.scopus.com/inward/record.url?scp=85164281384&partnerID=8YFLogxK
U2 - 10.1039/d3cp01655b
DO - 10.1039/d3cp01655b
M3 - Article
SN - 1463-9076
VL - 25
SP - 17943
EP - 17951
JO - Physical Chemistry Chemical Physics
JF - Physical Chemistry Chemical Physics
IS - 27
ER -