Identification of live and dead bacteria: A raman spectroscopic study

Runze Li; Dinesh Dhankhar; Jie Chen; Arjun Krishnamoorthi; Thomas C. Cesario; Peter M. Rentzepis

doi:10.1109/ACCESS.2019.2899006

Identification of live and dead bacteria: A raman spectroscopic study

Runze Li
, Dinesh Dhankhar
, Jie Chen
, Arjun Krishnamoorthi
, Thomas C. Cesario
, Peter M. Rentzepis

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Raman spectroscopy has been used to identify bacterial strains, bacterial components, such as protein and DNA bases, and the ratio of live to dead bacteria before and after exposure to ultraviolet (UV) radiation. New vibrational bands and changes in their intensity as a function of UV irradiation time have been recorded by high resolution Raman spectroscopy which made it possible to determine the mechanism of the UV inactivation of Escherichia coli (E. coli), Serratia marcescens (S. marcescens), and Micrococcus luteus (M. luteus) bacteria in saline solutions. We have also employed a novel, new, handheld spectrometer capable of recording, in situ, within minutes, the absorption, fluorescence, synchronous fluorescence, and Raman spectra of bacteria and other biological species and large molecules.

Original language	English
Article number	8641277
Pages (from-to)	23549-23559
Number of pages	11
Journal	IEEE Access
Volume	7
DOIs	https://doi.org/10.1109/ACCESS.2019.2899006
State	Published - 2019
Externally published	Yes

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10.1109/ACCESS.2019.2899006

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title = "Identification of live and dead bacteria: A raman spectroscopic study",

abstract = "Raman spectroscopy has been used to identify bacterial strains, bacterial components, such as protein and DNA bases, and the ratio of live to dead bacteria before and after exposure to ultraviolet (UV) radiation. New vibrational bands and changes in their intensity as a function of UV irradiation time have been recorded by high resolution Raman spectroscopy which made it possible to determine the mechanism of the UV inactivation of Escherichia coli (E. coli), Serratia marcescens (S. marcescens), and Micrococcus luteus (M. luteus) bacteria in saline solutions. We have also employed a novel, new, handheld spectrometer capable of recording, in situ, within minutes, the absorption, fluorescence, synchronous fluorescence, and Raman spectra of bacteria and other biological species and large molecules.",

author = "Runze Li and Dinesh Dhankhar and Jie Chen and Arjun Krishnamoorthi and Cesario, \{Thomas C.\} and Rentzepis, \{Peter M.\}",

year = "2019",

doi = "10.1109/ACCESS.2019.2899006",

language = "English",

volume = "7",

pages = "23549--23559",

journal = "IEEE Access",

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T1 - Identification of live and dead bacteria

T2 - A raman spectroscopic study

AU - Li, Runze

AU - Dhankhar, Dinesh

AU - Chen, Jie

AU - Krishnamoorthi, Arjun

AU - Cesario, Thomas C.

AU - Rentzepis, Peter M.

PY - 2019

Y1 - 2019

N2 - Raman spectroscopy has been used to identify bacterial strains, bacterial components, such as protein and DNA bases, and the ratio of live to dead bacteria before and after exposure to ultraviolet (UV) radiation. New vibrational bands and changes in their intensity as a function of UV irradiation time have been recorded by high resolution Raman spectroscopy which made it possible to determine the mechanism of the UV inactivation of Escherichia coli (E. coli), Serratia marcescens (S. marcescens), and Micrococcus luteus (M. luteus) bacteria in saline solutions. We have also employed a novel, new, handheld spectrometer capable of recording, in situ, within minutes, the absorption, fluorescence, synchronous fluorescence, and Raman spectra of bacteria and other biological species and large molecules.

AB - Raman spectroscopy has been used to identify bacterial strains, bacterial components, such as protein and DNA bases, and the ratio of live to dead bacteria before and after exposure to ultraviolet (UV) radiation. New vibrational bands and changes in their intensity as a function of UV irradiation time have been recorded by high resolution Raman spectroscopy which made it possible to determine the mechanism of the UV inactivation of Escherichia coli (E. coli), Serratia marcescens (S. marcescens), and Micrococcus luteus (M. luteus) bacteria in saline solutions. We have also employed a novel, new, handheld spectrometer capable of recording, in situ, within minutes, the absorption, fluorescence, synchronous fluorescence, and Raman spectra of bacteria and other biological species and large molecules.

UR - https://www.scopus.com/pages/publications/85062686728

U2 - 10.1109/ACCESS.2019.2899006

DO - 10.1109/ACCESS.2019.2899006

M3 - Article

VL - 7

SP - 23549

EP - 23559

JO - IEEE Access

JF - IEEE Access

M1 - 8641277

ER -

Identification of live and dead bacteria: A raman spectroscopic study

Abstract

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