Self-assembled, mesoporous polymeric networks for patterned protein arrays

Dolly Batra; Stefan Vogt; Phillip D. Laible; Millicent Anne Firestone

doi:10.1021/la051948f

Self-assembled, mesoporous polymeric networks for patterned protein arrays

Dolly Batra
, Stefan Vogt
, Phillip D. Laible
, Millicent Anne Firestone

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A facile, self-assembly approach to the fabrication of a robust, mesoporous, biocompatible polymeric network for the spatial organization of proteins is described. Surface-deposited poly(styrene) (PS) beads that assemble into a two-dimensional (2-D) hexagonal array are used to template cross-linked poly(vinyl alcohol) (PVA), yielding an inverse opal structure. The porous, water insoluble network is used to entrain a model, soluble protein, green fluorescent protein (GFP). The polymeric network is characterized by atomic force microscopy (AFM) and optical microscopy, and the spatial localization of the incorporated GFP is determined by fluorescence microscopy. The results demonstrate that this system may constitute a versatile platform for the lateral organization of biomolecules.

Original language	English
Pages (from-to)	10301-10306
Number of pages	6
Journal	Langmuir
Volume	21
Issue number	23
DOIs	https://doi.org/10.1021/la051948f
State	Published - Nov 8 2005
Externally published	Yes

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10.1021/la051948f

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title = "Self-assembled, mesoporous polymeric networks for patterned protein arrays",

abstract = "A facile, self-assembly approach to the fabrication of a robust, mesoporous, biocompatible polymeric network for the spatial organization of proteins is described. Surface-deposited poly(styrene) (PS) beads that assemble into a two-dimensional (2-D) hexagonal array are used to template cross-linked poly(vinyl alcohol) (PVA), yielding an inverse opal structure. The porous, water insoluble network is used to entrain a model, soluble protein, green fluorescent protein (GFP). The polymeric network is characterized by atomic force microscopy (AFM) and optical microscopy, and the spatial localization of the incorporated GFP is determined by fluorescence microscopy. The results demonstrate that this system may constitute a versatile platform for the lateral organization of biomolecules.",

author = "Dolly Batra and Stefan Vogt and Laible, \{Phillip D.\} and Firestone, \{Millicent Anne\}",

year = "2005",

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AU - Vogt, Stefan

AU - Laible, Phillip D.

AU - Firestone, Millicent Anne

PY - 2005/11/8

Y1 - 2005/11/8

N2 - A facile, self-assembly approach to the fabrication of a robust, mesoporous, biocompatible polymeric network for the spatial organization of proteins is described. Surface-deposited poly(styrene) (PS) beads that assemble into a two-dimensional (2-D) hexagonal array are used to template cross-linked poly(vinyl alcohol) (PVA), yielding an inverse opal structure. The porous, water insoluble network is used to entrain a model, soluble protein, green fluorescent protein (GFP). The polymeric network is characterized by atomic force microscopy (AFM) and optical microscopy, and the spatial localization of the incorporated GFP is determined by fluorescence microscopy. The results demonstrate that this system may constitute a versatile platform for the lateral organization of biomolecules.

AB - A facile, self-assembly approach to the fabrication of a robust, mesoporous, biocompatible polymeric network for the spatial organization of proteins is described. Surface-deposited poly(styrene) (PS) beads that assemble into a two-dimensional (2-D) hexagonal array are used to template cross-linked poly(vinyl alcohol) (PVA), yielding an inverse opal structure. The porous, water insoluble network is used to entrain a model, soluble protein, green fluorescent protein (GFP). The polymeric network is characterized by atomic force microscopy (AFM) and optical microscopy, and the spatial localization of the incorporated GFP is determined by fluorescence microscopy. The results demonstrate that this system may constitute a versatile platform for the lateral organization of biomolecules.

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EP - 10306

JO - Langmuir

JF - Langmuir

IS - 23

ER -

Self-assembled, mesoporous polymeric networks for patterned protein arrays

Abstract

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