Surface-immobilized antibody-antigen binding studies by single molecule fluorescence imaging

Jamshid Temirov; Andrew Bradbury; James Henry Werner; Jamshid Temirov

doi:10.1117/12.645334

Surface-immobilized antibody-antigen binding studies by single molecule fluorescence imaging

Jamshid Temirov
, Andrew Bradbury
, James Henry Werner
, Jamshid Temirov

Los Alamos National Laboratory

Research output: Contribution to journal › Conference article

2 Scopus citations

Abstract

In this work we report Single Molecule (SM) microscopy studies of binding events between individual surface-immobilized antibodies and fluorescent antigens using Total Internal Reflection (TIR) microscopy. Specific binding events of single biotin-conjugated ¹quantum dots to anti-biotin antibodies, immobilized on an amine-coated cover glass using a heterobifunctional photo-reactive cross-linker were observed. The methodology of calculating the binding affinity (dissociation constant) from the time series of 2-D images is described.

Original language	English
Article number	60920O
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6092
DOIs	https://doi.org/10.1117/12.645334
State	Published - Jun 29 2006
Event	Ultrasensitive and Single-Molecule Detection Technologies - Duration: Jun 29 2006 → …

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title = "Surface-immobilized antibody-antigen binding studies by single molecule fluorescence imaging",

abstract = "In this work we report Single Molecule (SM) microscopy studies of binding events between individual surface-immobilized antibodies and fluorescent antigens using Total Internal Reflection (TIR) microscopy. Specific binding events of single biotin-conjugated 1quantum dots to anti-biotin antibodies, immobilized on an amine-coated cover glass using a heterobifunctional photo-reactive cross-linker were observed. The methodology of calculating the binding affinity (dissociation constant) from the time series of 2-D images is described.",

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AU - Temirov, Jamshid

AU - Bradbury, Andrew

AU - Werner, James Henry

AU - Temirov, Jamshid

PY - 2006/6/29

Y1 - 2006/6/29

N2 - In this work we report Single Molecule (SM) microscopy studies of binding events between individual surface-immobilized antibodies and fluorescent antigens using Total Internal Reflection (TIR) microscopy. Specific binding events of single biotin-conjugated 1quantum dots to anti-biotin antibodies, immobilized on an amine-coated cover glass using a heterobifunctional photo-reactive cross-linker were observed. The methodology of calculating the binding affinity (dissociation constant) from the time series of 2-D images is described.

AB - In this work we report Single Molecule (SM) microscopy studies of binding events between individual surface-immobilized antibodies and fluorescent antigens using Total Internal Reflection (TIR) microscopy. Specific binding events of single biotin-conjugated 1quantum dots to anti-biotin antibodies, immobilized on an amine-coated cover glass using a heterobifunctional photo-reactive cross-linker were observed. The methodology of calculating the binding affinity (dissociation constant) from the time series of 2-D images is described.

UR - https://www.scopus.com/pages/publications/33745289417

U2 - 10.1117/12.645334

DO - 10.1117/12.645334

M3 - Conference article

SN - 1605-7422

VL - 6092

JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

M1 - 60920O

T2 - Ultrasensitive and Single-Molecule Detection Technologies

Y2 - 29 June 2006

ER -

Surface-immobilized antibody-antigen binding studies by single molecule fluorescence imaging

Abstract

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