Three dimensional time-gated tracking of non-blinking quantum dots in live cells

Matthew S. DeVore; Dominik G. Stich; Aaron M. Keller; Yagnaseni Ghosh; Peter M. Goodwin; Mary E. Phipps; Michael H. Stewart; Cédric Cleyrat; Bridget S. Wilson; Diane S. Lidke; Jennifer A. Hollingsworth; James H. Werner

doi:10.1117/12.2082943

Three dimensional time-gated tracking of non-blinking quantum dots in live cells

Matthew S. DeVore
, Dominik G. Stich
, Aaron M. Keller
, Yagnaseni Ghosh
, Peter M. Goodwin
, Mary E. Phipps
, Michael H. Stewart
, Cédric Cleyrat
, Bridget S. Wilson
, Diane S. Lidke
, Jennifer A. Hollingsworth
, James H. Werner

Los Alamos National Laboratory

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

8 Scopus citations

Abstract

Single particle tracking has provided a wealth of information about biophysical processes such as motor protein transport and diffusion in cell membranes. However, motion out of the plane of the microscope or blinking of the fluorescent probe used as a label generally limits observation times to several seconds. Here, we overcome these limitations by using novel non-blinking quantum dots as probes and employing a custom 3D tracking microscope to actively follow motion in three dimensions (3D) in live cells. Signal-to-noise is improved in the cellular milieu through the use of pulsed excitation and time-gated detection.

Original language	English
Title of host publication	Colloidal Nanoparticles for Biomedical Applications X
Editors	Wolfgang J. Parak, Marek Osinski, Xing-Jie Liang
Publisher	Unknown Publisher
ISBN (Electronic)	9781628414288
DOIs	https://doi.org/10.1117/12.2082943
State	Published - Jan 1 2015
Event	Colloidal Nanoparticles for Biomedical Applications X - Duration: Jan 1 2015 → …

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	9338
ISSN (Print)	1605-7422

Conference

Conference	Colloidal Nanoparticles for Biomedical Applications X
Period	01/1/15 → …

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10.1117/12.2082943

Cite this

DeVore, M. S., Stich, D. G., Keller, A. M., Ghosh, Y., Goodwin, P. M., Phipps, M. E., Stewart, M. H., Cleyrat, C., Wilson, B. S., Lidke, D. S., Hollingsworth, J. A., & Werner, J. H. (2015). Three dimensional time-gated tracking of non-blinking quantum dots in live cells. In W. J. Parak, M. Osinski, & X.-J. Liang (Eds.), Colloidal Nanoparticles for Biomedical Applications X Article 933812 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9338). Unknown Publisher. https://doi.org/10.1117/12.2082943

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title = "Three dimensional time-gated tracking of non-blinking quantum dots in live cells",

abstract = "Single particle tracking has provided a wealth of information about biophysical processes such as motor protein transport and diffusion in cell membranes. However, motion out of the plane of the microscope or blinking of the fluorescent probe used as a label generally limits observation times to several seconds. Here, we overcome these limitations by using novel non-blinking quantum dots as probes and employing a custom 3D tracking microscope to actively follow motion in three dimensions (3D) in live cells. Signal-to-noise is improved in the cellular milieu through the use of pulsed excitation and time-gated detection.",

author = "DeVore, \{Matthew S.\} and Stich, \{Dominik G.\} and Keller, \{Aaron M.\} and Yagnaseni Ghosh and Goodwin, \{Peter M.\} and Phipps, \{Mary E.\} and Stewart, \{Michael H.\} and C{\'e}dric Cleyrat and Wilson, \{Bridget S.\} and Lidke, \{Diane S.\} and Hollingsworth, \{Jennifer A.\} and Werner, \{James H.\}",

year = "2015",

month = jan,

day = "1",

doi = "10.1117/12.2082943",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "Unknown Publisher",

editor = "Parak, \{Wolfgang J.\} and Marek Osinski and Xing-Jie Liang",

booktitle = "Colloidal Nanoparticles for Biomedical Applications X",

note = "Colloidal Nanoparticles for Biomedical Applications X ; Conference date: 01-01-2015",

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DeVore, MS, Stich, DG, Keller, AM, Ghosh, Y, Goodwin, PM, Phipps, ME, Stewart, MH, Cleyrat, C, Wilson, BS, Lidke, DS, Hollingsworth, JA & Werner, JH 2015, Three dimensional time-gated tracking of non-blinking quantum dots in live cells. in WJ Parak, M Osinski & X-J Liang (eds), Colloidal Nanoparticles for Biomedical Applications X., 933812, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9338, Unknown Publisher, Colloidal Nanoparticles for Biomedical Applications X, 01/1/15. https://doi.org/10.1117/12.2082943

Three dimensional time-gated tracking of non-blinking quantum dots in live cells. / DeVore, Matthew S.; Stich, Dominik G.; Keller, Aaron M. et al.
Colloidal Nanoparticles for Biomedical Applications X. ed. / Wolfgang J. Parak; Marek Osinski; Xing-Jie Liang. Unknown Publisher, 2015. 933812 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9338).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Goodwin, Peter M.

AU - Phipps, Mary E.

AU - Stewart, Michael H.

AU - Cleyrat, Cédric

AU - Wilson, Bridget S.

AU - Lidke, Diane S.

AU - Hollingsworth, Jennifer A.

AU - Werner, James H.

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AB - Single particle tracking has provided a wealth of information about biophysical processes such as motor protein transport and diffusion in cell membranes. However, motion out of the plane of the microscope or blinking of the fluorescent probe used as a label generally limits observation times to several seconds. Here, we overcome these limitations by using novel non-blinking quantum dots as probes and employing a custom 3D tracking microscope to actively follow motion in three dimensions (3D) in live cells. Signal-to-noise is improved in the cellular milieu through the use of pulsed excitation and time-gated detection.

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M3 - Conference contribution

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Colloidal Nanoparticles for Biomedical Applications X

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A2 - Osinski, Marek

A2 - Liang, Xing-Jie

PB - Unknown Publisher

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ER -

DeVore MS, Stich DG, Keller AM, Ghosh Y, Goodwin PM, Phipps ME et al. Three dimensional time-gated tracking of non-blinking quantum dots in live cells. In Parak WJ, Osinski M, Liang XJ, editors, Colloidal Nanoparticles for Biomedical Applications X. Unknown Publisher. 2015. 933812. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2082943

Three dimensional time-gated tracking of non-blinking quantum dots in live cells

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