Time-resolved super-resolution microscopy to image photoluminescence lifetimes and spatially resolve dual-emitter semiconductor nanostructures

Megan K. Dunlap, Liam A. Koch, Duncan P. Ryan, Peter M. Goodwin, James H. Werner, Paul B. Bourdin, Jennifer A. Hollingsworth, Chris J. Sheehan, Martin P. Gelfand, Alan Van Orden

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

A time-dependent likelihood distribution for analyzing time correlated single photon counting data from a four-pixel time-resolved single molecule localization microscopy experiment is discussed. It is generated by accounting for the probabilities to record photons from two emitters, background counts, and dark counts during two different time channels relative to each incident laser pulse in the experiment. Maximizing the distribution enables localization of each emitter in a dual emitting nanostructure based on the disparate photoluminescence lifetimes of the emitters, even when both emitters are simultaneously in an emissive state. The technique is demonstrated using simulated photon counting data from a hypothetical non-blinking dual-emitter nanostructure in which the distance between the two emitters is less than 10-nm.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Superresolution Imaging XVI
EditorsIngo Gregor, Felix Koberling, Rainer Erdmann
PublisherUnknown Publisher
ISBN (Electronic)9781510658776
DOIs
StatePublished - 2023
EventSingle Molecule Spectroscopy and Superresolution Imaging XVI 2023 -
Duration: Jan 1 2023 → …

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume12386
ISSN (Print)1605-7422

Conference

ConferenceSingle Molecule Spectroscopy and Superresolution Imaging XVI 2023
Period01/1/23 → …

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